Research Articles
Genome Editing in Plants: A Comprehensive Comparison of CRISPR-Cas9, TALEN, and ZFN Efficiency, Specificity, and Applications
This article provides a systematic analysis of the three major genome-editing platforms—ZFNs, TALENs, and CRISPR-Cas9—in plant systems.
The CRISPR-Cas9 System Decoded: A Comprehensive Guide to sgRNA, Cas9, and Their Applications in Biomedical Research
This article provides a complete overview of the CRISPR-Cas9 system, focusing on the core components—the Cas9 nuclease and single-guide RNA (sgRNA).
NHEJ vs HDR in Plants: Mastering DNA Repair Mechanisms for Precision Genome Editing and Crop Improvement
This article provides a comprehensive analysis of the non-homologous end joining (NHEJ) and homology-directed repair (HDR) pathways in plant cells, crucial mechanisms for maintaining genomic integrity and enabling advanced biotechnology.
Protoplast CRISPR Delivery: Methods, Optimization, and Applications in Plant Genome Engineering
This article provides a comprehensive overview of CRISPR-Cas9 delivery methods utilizing plant protoplasts, a key platform for DNA-free genome editing.
Cross-Species Hormonal Profiling by LC-MS/MS: Methodologies, Applications, and Advances in Biomedical Research
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has emerged as the gold standard for the simultaneous quantification of multiple hormone classes across diverse species, overcoming the limitations of traditional immunoassays.
Building Robust Data Preprocessing Pipelines for Quantitative Plant Phenotyping: From Raw Data to Actionable Insights
This article provides a comprehensive guide to constructing effective data preprocessing pipelines specifically for quantitative plant data analysis.
Systems Biology of Plant Robustness: From Molecular Networks to Climate Resilience
This article synthesizes current systems biology research on plant robustness—the ability to maintain function amidst genetic, environmental, and pathogenic perturbations.
Optimizing Split-Root Assays: A Strategic Guide to Enhancing Replicability and Robustness in Plant Science Research
This article provides a comprehensive framework for optimizing split-root assays, a crucial technique for studying systemic signaling and plant responses to heterogeneous environments.
Evolutionary Trade-Offs in Plants: Unraveling the Mechanisms of Robustness Versus Efficiency for Future Crops and Biomedical Insights
This review synthesizes contemporary research on the fundamental evolutionary trade-offs between robustness—the capacity to buffer phenotypes against genetic and environmental perturbations—and efficiency in plants.
Enhancing Gene Function Studies: How the Truncated CMV C2bN43 Suppressor Optimizes VIGS Technology
This article explores a significant advancement in Virus-Induced Gene Silencing (VIGS), a key technique for determining gene function in plants.